Part:BBa_K4245135
Lettuce Right
This part is the right component of the split fluorescent DNA aptamer Lettuce (BBa_K4245133), developed by the Weill-Cornell Medical College. Lettuce right is a 26 nucleotide long DNA aptamer that, when brought in close proximity to Lettuce left (BBa_K4245134), induces fluorescence of a GFP fluorophore mimic (Z)-4-(3,5-difluoro-4-hydroxybenzylidene)-2-methyl-1-(2,2,2-trifluoroethyl)-1H-imidazol-5(4 H)-one (DFHBI-1T) (VarnBuhler et al., 2022).
The mechanism in which the split Lettuce aptamer works is represented in Figure 1.
References
VarnBuhler, B. S., Moon, J., Dey, S. K., Wu, J., & Jaffrey, S. R. (2022). Detection of SARS-CoV-2 RNA using a DNA aptamer mimic of Green Fluorescent Protein. ACS Chemical Biology, 17(4), 840–853. https://doi.org/10.1021/acschembio.1c00893
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 9
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 9
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 9
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 9
- 1000COMPATIBLE WITH RFC[1000]
None |